50pcs Cellulose acetate film Electrophoretic membrane 2*8cm 8*12cm

Description: This auction is for: NEW 50pcs Cellulose acetate film Electrophoretic membrane Descriptionjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjj · Material: Cellulose acetate · size: 2cm*8cm/8cm*12cm· Quantity: 50pcs Cellulose acetate film has a low adsorption capacity for protein samples and can almost completely eliminate the "tailing" phenomenon that occurs in paper electrophoresis. Additionally, due to the relatively small hydrophilicity of the film, it contains less buffer and most of the current during electrophoresis is conducted by the sample, resulting in fast separation speed, short electrophoresis time, and low sample consumption μ G protein can achieve satisfactory separation results. Therefore, it is particularly suitable for the detection of trace abnormal proteins in pathological situations. Compared with filter paper, cellulose acetate film has the following advantages: (1) Good separation effect. There is minimal adsorption on protein samples and no "tailing" phenomenon. After staining, the background can be completely decolorized, and various protein staining bands are separated clearly, thus improving the accuracy of quantitative determination. (2) Fast and time-saving. Due to its smaller hydrophilicity compared to filter paper and less electroosmotic effect, most of the current during electrophoresis is conducted by the sample, resulting in fast separation speed and short electrophoresis time. Generally, electrophoresis takes only 45-60 minutes. In addition to staining and decolorization, the entire electrophoresis process only takes about 90 minutes to complete. (3) High sensitivity, low sample usage, serum protein electrophoresis only requires 2 μ Serum is often used to detect changes in trace abnormal proteins under pathological conditions. (4) Widely applied. Some proteins are difficult to separate on paper electrophoresis, such as fetal alpha globulin, lysozyme, insulin, histones, etc., which can be well separated using cellulose acetate film electrophoresis. (5) Easy to store and quantify. After electrophoresis staining of cellulose acetate film, it can be soaked in a mixture of glacial acetic acid, ethanol or other solutions to make a transparent dry plate, which is conducive to scanning quantification and long-term preservation. After being treated with glacial acetic acid ethanol solution or other transparent solution, the cellulose acetate membrane can be made transparent, which is beneficial for the determination of electrophoretic spectra by light absorption scanning and the long-term preservation of the membrane. 1. The commonly used electrophoresis buffer is a barbiturate buffer with a pH of 8.6, with a concentration of 0.05-0.09 mol/L. 2. Key points of operation (1) Membrane pre-treatment: The membrane must be soaked in buffer solution before electrophoresis. After soaking, the membrane should be removed and excess buffer solution should be sucked away with filter paper, and should not be too dry. And mark one corner. (2) Sample addition: The amount of sample used depends on factors such as sample concentration, intrinsic properties, staining method, and detection method. Routine electrophoresis analysis of serum proteins, with no more than 1 sample line added per cm μ l. Equivalent to 60-80 μ G protein. (3) Electrophoresis: Can be performed at room temperature. The voltage is 25 V/cm, and the current is 0.4~0.6 mA/cm wide. (4) Staining: Generally, amino black and ponceau red are used for protein staining, toluidine blue or periodate Schiff reagent is used for glycoproteins, and * * * black or magenta sulfite is used for lipoprotein staining. (5) Bleaching and transparency: The most commonly used bleaching agent for water-soluble dyes is a 5% acetic acid aqueous solution. For long-term preservation or light absorption scanning measurement, it can be immersed in a transparent solution of glacial acetic acid: anhydrous ethanol=30:70 (V/V). Shipping Jjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjj Due to the global pandemic, the deliver time will be longer than normal time, the deliver speed is slower and can't control.We send item via register airmail to worldwide(speedpak, epacket, post office airmail). comes with tracking NO., Your item will be shipped out within 48 hours after your payment clears. Please note some seller from Hongkong or south of China send goods via Hongkong airmail,it can arrive many country within 10-15 days, we are in east of China,only can use china airmail to send the goods,it is slower than Hongkong airmail,please check the form as follows to see the detail normal deliver time,if you can't wait that time, please don't purchase the items from us. Country NameNormal Deliver TimeTo North America14-28 business days To Most West/North Europe country14-28 business daysTo East/Middle Europe country21-35 business daysTo South America30-50 business daysTo Australia/New Zealand14-28 business days To East Asia 14-25 business daysTo West /Middle Asia21-35 business daysTo Russia21-35 business days TO Africa 50-70 business days Remark:The delivery time above will be excluded from there are local largeholiday(such as Xmas)or the local strike, if similar factors exist, deliver time will be longer than this, the goods may be delayed. Import duties, taxes and charges are not included in the item price or shipping charges. These charges are the buyer's responsibility. PaymentJjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjj We accept Paypal ExchangeJjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjj Unsatisfied product can be returned within 14 days from receiving your item. We issue immediate full refund minus all shipping costs. New replacement item will be shipped out as soon as defective item received.

Price: 25.99 USD

Location: shanghai

End Time: 2023-11-20T09:10:05.000Z

Shipping Cost: 0 USD