Notch Signaling Research: Methods and Protocols by Dongyu Jia (English) Hardcove

Description: Notch Signaling Research by Dongyu Jia The collected research methods and protocols include the dissection of Notch functional sites, Notch regulators, Notch activity reporters and analysis, roles of Notch in development and diseases, wet and dry lab tools and studies, and bioinformatics analysis. FORMAT Hardcover LANGUAGE English CONDITION Brand New Publisher Description This detailed volume explores the large research area of Notch signaling studies in a variety of model organisms. The collected research methods and protocols include the dissection of Notch functional sites, Notch regulators, Notch activity reporters and analysis, roles of Notch in development and diseases, wet and dry lab tools and studies, and bioinformatics analysis. This book particularly emphasizes on the exploration of Notch roles in development and diseases, while acknowledging the importance of combining classic and novel molecular tools and methods, bioinformatics workflows and applications, and different model organisms to fully understand the subject area. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Notch Signaling Research: Methods and Protocols is an ideal guide for researchers in the field, with techniques that could potentially be applied to study the wider world of other signaling pathways as well. Back Cover This detailed volume explores the large research area of Notch signaling studies in a variety of model organisms. The collected research methods and protocols include the dissection of Notch functional sites, Notch regulators, Notch activity reporters and analysis, roles of Notch in development and diseases, wet and dry lab tools and studies, and bioinformatics analysis. This book particularly emphasizes on the exploration of Notch roles in development and diseases, while acknowledging the importance of combining classic and novel molecular tools and methods, bioinformatics workflows and applications, and different model organisms to fully understand the subject area. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Notch Signaling Research: Methods and Protocols is an ideal guide for researchers in the field, with techniques that could potentially be applied to study the wider world of other signaling pathways as well. Table of Contents Using the CRISPR/Cas9 System for Dissection of Functional Sites of the Notch Gene in Drosophila melanogaster.- Screening Mutants by Single Fly Genomic PCR.- Generation of Properly Folded Epidermal Growth Factor-Like (EGF) Repeats and Glycosyltransferases Enables In Vitro O-Glycosylation.- Use of FLP/FRT System to Screen for Notch Signaling Regulators in the Drosophila Wing.- High-Throughput Analysis to Identify Activators of Notch Signaling.- Artificial Notch Signaling Activation Method Using Immobilized Ligand Beads.- Mammalian NOTCH Receptors Activation and Signaling Protocols.- Somatic Clonal Analyses Using FLP/FRT and MARCM System to Understand Notch Signaling Mechanism and Its Regulation.- Analyzing the Interaction of RBPJ with Mitotic Chromatin and Its Impact on Transcription Reactivation upon Mitotic Exit.- Assessing the Roles of Potential Notch Signaling Components in Instructive and Permissive Pathways with Two Drosophila Pericardial Reporters.- Image-Based Single-Molecule Analysis of Notch-Dependent Transcription in Its Natural Context.- An Automatic Stage Identification MATLAB Tool to Reveal Notch Expression Pattern in Drosophila Egg Chambers.- Employing the CRISPR Technology for Studying Notch Signaling in the Male Gonad of Drosophila melanogaster.- Immuno-Localization of Notch Signaling in Mouse Preimplantation Embryos.- Studying the NOTCH Signaling Pathway Activation in Kidney Biopsies.- Exosomes as Carriers for Notch Molecules.- In Vivo and Ex Vivo Experimental Approach for Studying Functional Role of Notch in Pulmonary Vascular Disease.- Metastasis Model to Test the Role of Notch Signaling in Prostate Cancer.- Functional Studies of Genetic Variants Associated with Human Diseases in Notch Signaling-Related Genes Using Drosophila.- Bioinformatics Tools to Understand Notch. Feature Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts Details ISBN1071622005 Short Title Notch Signaling Research Series Methods in Molecular Biology Language English Year 2022 ISBN-10 1071622005 ISBN-13 9781071622001 Format Hardcover Subtitle Methods and Protocols Series Number 2472 Publisher Springer-Verlag New York Inc. Edition 1st Imprint Springer-Verlag New York Inc. Place of Publication New York, NY Country of Publication United States Pages 298 Publication Date 2022-06-09 AU Release Date 2022-06-09 NZ Release Date 2022-06-09 US Release Date 2022-06-09 UK Release Date 2022-06-09 Author Dongyu Jia Edited by Dongyu Jia Illustrations 45 Illustrations, color; 11 Illustrations, black and white; XIII, 298 p. 56 illus., 45 illus. in color. With online files/update. Edition Description 1st ed. 2022 Alternative 9781071622032 DEWEY 571.74 Audience Professional & Vocational We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! TheNile_Item_ID:135423478;

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